The thermo scientific pierce ecl plus substrate is a chemiluminescent and chemifluorescent peroxidase substrate for western blotting. Sensitive, long lasting substrate for chemiluminescent western blotting with a strong. Thermo scientific pierce antibodies are developed for. Chemiluminescent substrates for western blotting sigma. The ecl western blotting substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence. Alkaline phosphatase ap and horseradish peroxidase hrp substrates for use with film and ccd imaging systems, listed in order of increasing sensitivity. Tanon highsig ecl western blotting substrate is a highly sensitive nonradioactive, enhanced luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp on immunoblots. Pierce ecl plus western blotting substrate thermo fisher. Western blotting is easy with thermo scientific products pdf. Thermo scientific pierce fast western blot kit, ecl substrate contains optimized reagents that shorten the time to perform a typical western blot from 4 hours down to approximately 55 minutes.
Samples were transferred onto pvdf membrane and probed with 0. For detection with ecl plus when using hybond ecl membrane we have found in our laboratories that dilutions of 1. Each westar substrate is at the top of its respective market segment regarding performanceprice ratio. Westernbright ecl is a horseradish peroxidase substrate optimized for chemiluminescent western blots imaged using xray film. Supersignal west femto maximum sensitivity substrate. The boster western blotting substrate is a luminolbased chemiluminescent substrate and it can detect horseradish peroxidase hrp at high sensitivity levels low picogram. Western blotting also called immunoblotting, because an antibody is used to. I had the opportunity to use pierces ecl reagent for viewing proteins which reacted with the primary antibody sc95555, against clara cell protein cc10, from santa cruz biotechnology. Supersignal west femto maximum sensitivity substrate is designed for low femtogramlevel detection by western blot analysis. Pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray film or ccd or.
It is a chemiluminescent detection reagent in the amersham ecl product family that is recommended. Pierce ecl plus substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from hrpconjugated antibodies and probes by western blotting using xray film, ccd camera documentation systems or laserbased imagers. Supersignal west pico plus chemiluminescent substrate pub. Ecl western blotting substrate is a highly sensitive, nonradioactive, enhanced luminolbased chemiluminescent substrate for easy detection of horseradish peroxidase hrp on immunoblots. Instructions pierce fast western blot kit, ecl substrate.
Western blotting protocol using chemiluminescent substrates. Blots can be repeatedly exposed to xray film to obtain optimal results or stripped of the immunodetection reagents and reprobed. Usa 800 424 6723 australia 61 2 9914 2800 austria 01 877 89 01 belgium 09 385 55 11 brazil 55 11 5044 5699. Lumigen ecl plus is invented, developed and manufactured by lumigen. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard enhanced chemilumescent ecl substrates for detection of horseradish peroxidase hrp enzyme activity, yet costs much less. Ecl western blotting substrate kit k820 biovision, inc. The ecl western blotting substrate is a highly sensitive nonradioactive, enhanced luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp on immunoblots.
Because the luminol and peroxide reagentformulations are identical to other commercially available substrate. Clarity western ecl substrate, biorad pierce ecl, thermo scientific. Blots can be repeatedly exposed to xray film to obtain optimal results or stripped of the. Pi32106 pierce ecl western blotting substrate 500 ml kit. Western blotting luminol reagent is recommended for western blotting enhanced chemiluminescence applications using horseradish peroxidase hrp conjugated secondary antibodies. Ecl western blotting substrate is compatible with pvdf and nitrocellulose membranes and various blotting buffers. Thermo scientific pierce ecl 2 western blotting substrate flyer. Not recommended or intended for diagnosis of disease in humans. Detection reagent 1, peroxide solution not available. Immobilon western hrp substrates are a family of three premixed reagents for horseradish peroxidasebased detection that offer significant advantages over other. Clarity and clarity max ecl western blotting substrates life.
Thermo scientific pierce ecl western blotting substrate is a valuepriced, entrylevel horseradish peroxidase hrp substrate for enhanced chemiluminescence ecl that directly replaces costlier products without the need to reoptimize conditions. Pierce ecl plus western blotting substrate 25 ml pi80197 100 ml pi80196 3 x 100 ml pi80196x3 supersignal west pico plus substrate supersignal west pico plus chemiluminescent substrate 20 ml pi34579 200 ml pi34577 500 ml pi34580 1 l pi34578 description unit size cat. Thermo scientific pierce ecl western blotting substrate is a valuepriced, entrylevel peroxidase substrate for enhanced chemiluminescence ecl that directly replaces costlier products without the need to reoptimize conditions. The ecl plus western blotting detection reagents from amersham biosciences provide an improved nonradioactive method for the detection of immobilized. Hrp chemiluminescent substrates our signals are loud and. The thermo scientific pierce fast western blot kit, ecl substrate contains optimized reagents that shorten the time to perform a typical western blot from 4 hours to 55 minutes. Thermo scientific pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray. Use the same blotting conditions when switching from amersham ecl plus western blotting. Ecl western blotting substrate offers superior signal and clear background.
Enhanced chemiluminescence ecl is a highly sensitive method for detecting proteins bound to western blotting membranes. Single component, readytouse formulations simplify detection for steadfast reproducibility with enhanced. When combined with optimized antibody concentrations and blocking buffers, supersignal west femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ecl substrates. Westernbright ecl is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to. Hrp chemiluminescent substrates our signals are loud and clear. Pierce ecl substrate 42 supersignal chemiluminescent 4347 substrates and kits lumiphos chemiluminescent substrate 48 quick reference substrate guide 48 data imaging 49 thermo scienti. Chemiluminescent western blotting technical guide and protocols or our supersignal substrate western blotting handbook for a detailed western blotting protocol. Lowpicogram detection with supersignal west pico plus. Advanstas ecl western blotting reagent is optimized for xray film and digital. If you are currently using a supersignal chemiluminescent substrate, switching to tanon highsig ecl western blotting substrate requires increasing antigen and antibody concentrations. Thermo scientific pierce ecl plus substrate helasds595 1.
This substrate should not be in the light for a long time, please protect it from light, and manipulate in dark room. Thermo scientific pierce fast western blot kit, ecl. Amersham ecl start western blotting detection reagent amersham ecl start western blotting detection reagent fig 1 is a luminolbased chemiluminescent substrate designed for the detection of proteins immobilized to a membrane. Thermo scientific pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray film or ccd or laserbased imagers.
Western blotting the pierce way step 4b step 5 step 6 step 7 step 8 3 new. Westernbright ecl is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. Thermo scientific pierce fast western blot kit, ecl substrate. Pierce ecl plus western blotting substrate product number. Clarity and ecl plus provided the best signaltonoise ratios across all protein loads. Sep 25, 2007 while working at purdue university, one of the kits i used for detection of horseradish peroxidasewestern blots was pierces ecl substrate. Sustained luminescence signal is sustained for over. Chemiluminescent substrates for western blotting we offer a broad selection of ecl enhanced chemiluminescence substrates for every application. Bosters ecl plus western blotting substrate is an ultrasensitive, luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp. Detection of recombinant human crp visualized with visulite ecl western blotting substrate. Thermo scientific pierce ecl 2 western blotting substrate. Chemiluminescent substrates for western blotting sigmaaldrich.
High sensitivity ecl substrate kit ab3406 is designed for the detection of proteins with 23pg187ng of protein per band. Use the same blotting conditions when switching from amersham ecl substrate or pierce ecl substrate to tanon highsig ecl western blotting substrate. Because pierce ecl plus western blotting substrate is the same as amersham ecl plus western blotting detection reagent, there is no need for additional optimization of assay conditions when switching substrates. Tanon highsig ecl western blotting substrate enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods. For convenience and economy, pierce also offers complete western blotting kits thatinclude chemiluminescent substrates, enzymeconjugated antibodies, blocking buffers and standard buffers. The high sensitivity ecl substrate produces a strong signal with very low. Pierce ecl plus western blotting substrate fisher scientific. For example, our supersignal west pico plus substrate is recommended. Ecl western blotting detection reagents in combination with hybond ecl membranes form a complete solution for western blot applications.
A comparison between commercial and selfprepared ecl reagents. Increasing concentrations of protein adult testis lysate prepared in lysis buffer. Pierce ecl plus substrate can also be detected with certain phosphor imagers. Ecl plus supersignal west dura supersignal west pico ecl clarity ecl plus supersignal west dura supersignal west pico signaltonoise ratio ecl 70 60 50 40 30 20 10 0 10. Boster western blotting substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase hrpconjugates. Highly sensitive, nonradioactive, enhanced luminolbased chemiluminescent substrate for easy detection of horseradish peroxidase hrp on immunoblots. In order to obtain the best result, it is necessary to optimize the concentration of primary and second antibodies. Pi46428 restore plus western blot stripping buffer 30 ml suf. Plus you wont need to worry about reoptimizing your blots with a new substrate. Ecl plus western blotting reagent pack rpn 2124 before using this product, please read the instructions below for safe handling, storage and disposal. Western blotting handbook and troubleshooting guide. The ecl western blotting substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of hrp. While working at purdue university, one of the kits i used for detection of horseradish peroxidasewestern blots was pierces ecl substrate. If you are currently using a thermo scientific supersignal chemiluminescent substrate, switching to pierce ecl plus.
Ecl western blotting substrate from thermo scientific pierce. Pierce ecl plus substrate generates acridinium esters when it reacts with hrp. Thermo scientific pierce ecl plus western blotting substrate. Lumigen ecl plus ps3 is an acridanbased technology chemiluminescent and chemifluorescent substrate for sensitive detection of western blotted proteins that are bound to horseradish peroxidase hrp labeled antibodies. Pierce ecl 2 substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from hrpconjugated antibodies and probes by western blotting using xray film, ccd camera documentation systems or laserbased imagers. S outhern blot is used for transferring d na, n orthern blot for r na and w estern blot for p rotein. This product is sold as pierce ecl 2 substrate cat. Pi46430 restore plus western blot stripping buffer 500 ml suf. Pierce ecl plus western blotting substrate characteri. Enhanced chemiluminescence ecl for routine immunoblotting.
Thermo scientific pierce ecl 2 substrate is acridanbased, generating. Thermo scientific pierce ecl western blotting substrate. The kit provides all the reagents necessary to complete a western blot being probed with a mouse or rabbit primary antibody. Tanon highsig ecl western blotting substrate tanon. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with ccd imaging. Ecl western blotting detection substrate description note troubleshooting 1. Ecl western blotting detection reagents ge healthcare. Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration. The pierce ecl plus substrate generates acridinium esters when it reacts with horseradish peroxidase hrp, producing a strong signal that is equal to or better than similar commercially available substrates figure 1. Advansta westernbright ecl chemiluminescent hrp substrate. No single substrate performs best for all western blot systems and meets all sensitivity requirements. We offer a broad selection of ecl enhanced chemiluminescence substrates for every application. The term blotting refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane.
Amersham ecl western blotting detection reagents for 4000 cm2 membrane rpn2106. Western blotting is easy with thermo scientific products. Pierce ecl plus western blotting substrate thermo fisher scientific. Sensitivity requirements for western blotting may vary depending on the abundance of the target protein, the sample availability, and the quality of the antibodies. Table of contents page product overview 2 product structure and generation of chemiluminescent signal 2. Lumigen ecl plus ps3 is an acridanbased technology chemiluminescent and chemifluorescent substrate for sensitive detection of western blotted proteins that are bound to horseradish peroxidase hrp labeled antibodies highly sensitive approximately 5 pg per band of target protein on western blot.
One western blotting luminol reagent package contains 125 ml each solution a and solution b, which combine to form sufficient chemiluminescence reagent for a 2,000 cm. Clarity and clarity max ecl western blotting substrates. Ecl western blotting substrate infinity ecl detection reagents are nonradioactive, luminolbased chemiluminescent substrates, developed for the detection of immobilized proteins and nucleic acids conjugated with horseradishperoxidase hrp. Ecl plus, dilution factors may need to be greater than previously used with other detection systems. Biorads clarity family of ecl substrates provides simple, highperformance solutions for all your western blotting needs. Western blot results require optimizing the process components and steps. Thermo scientific pierce antibodies are developed for a wide variety of application needs. Amersham ecl western blotting detection reagents for.
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